This chemical is primarily used in the field of peptide synthesis, where it serves as a protected amino acid derivative. It is particularly valuable in solid-phase peptide synthesis (SPPS) due to the presence of the fluorenylmethoxycarbonyl (Fmoc) protecting group, which can be selectively removed under basic conditions without affecting other sensitive functional groups. The incorporation of the 2,4-difluorophenyl moiety enhances the compound's utility in designing peptides with specific binding properties or biological activities, often targeting enzymes or receptors where fluorine substitution can improve stability and interaction. Additionally, it is employed in the development of pharmaceuticals and bioactive peptides, especially in research focused on creating compounds with antimicrobial, antiviral, or anticancer properties. Its role in peptide modification and drug discovery makes it a critical reagent in medicinal chemistry and biochemistry.