This chemical is primarily used in peptide synthesis, particularly in the field of biochemical research. It serves as a valuable tool for studying enzyme activity and protein interactions due to its fluorescent properties. The EDANS group acts as a fluorophore, enabling the monitoring of peptide cleavage or binding events in real-time. It is often employed in fluorescence resonance energy transfer (FRET) assays to investigate protease activity or to develop biosensors. Additionally, it is utilized in the synthesis of labeled peptides for diagnostic and therapeutic research, aiding in the understanding of biological pathways and molecular mechanisms. Its compatibility with solid-phase peptide synthesis makes it a versatile reagent in the development of novel biomolecules.