Used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) as a building block. It protects the thiol group of homocysteine, preventing unwanted side reactions during peptide chain assembly. The Fmoc group is removable under basic conditions, while the Trt group can be cleaved under acidic conditions, allowing selective deprotection. This compound is essential for synthesizing peptides containing homocysteine residues, which can be used to form disulfide bonds or for specific functional modifications, stabilizing protein structures. It is widely applied in pharmaceutical research, biotechnology, and the development of therapeutic peptides.