Z-Tyr-OtBu is widely used in peptide synthesis as a protecting group for tyrosine. It helps in preventing unwanted side reactions during the formation of peptide bonds, ensuring the integrity of the peptide chain. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it allows for the selective deprotection and coupling of amino acids. Its stability under various reaction conditions makes it a reliable choice for researchers developing complex peptides for pharmaceutical and biochemical studies. Additionally, Z-Tyr-OtBu is employed in the preparation of peptide-based drugs, where precise control over amino acid incorporation is critical for achieving desired biological activity.