This compound is primarily used in peptide synthesis as a protected form of lysine. The Boc (tert-butoxycarbonyl) group safeguards the amino group, while the OtBu (tert-butyl ester) protects the carboxyl group, ensuring selective reactions during peptide chain assembly. It is particularly valuable in solid-phase peptide synthesis (SPPS) and solution-phase synthesis, where controlled deprotection steps are essential. Its application is crucial in producing peptides for pharmaceutical research, drug development, and biochemical studies, enabling the creation of complex peptide structures with high precision. Additionally, it is utilized in the synthesis of modified peptides and peptidomimetics, which are important in designing therapeutic agents targeting specific biological pathways.