This compound is primarily used in peptide synthesis, where it serves as a protected amino acid derivative. The fluorenylmethoxycarbonyl (Fmoc) group acts as a protecting group for the amino functionality, preventing unwanted reactions during the peptide assembly process. Its chloroindole moiety makes it particularly useful in synthesizing peptides that require specific indole-containing residues, often found in biologically active peptides or proteins. The compound is carefully deprotected after peptide chain elongation to yield the desired peptide sequence. Its application is crucial in pharmaceutical research and development, especially in creating peptide-based drugs or studying protein interactions.