Used in peptide synthesis as a chiral building block, particularly in the preparation of fluorinated analogs of biologically active peptides. The 3,5-difluorophenyl group enhances metabolic stability and membrane permeability, making it valuable in pharmaceutical research for developing protease inhibitors and receptor ligands. The Fmoc group allows for solid-phase synthesis, enabling stepwise assembly of peptides under mild basic conditions. Its stereochemistry is critical for maintaining biological activity in target molecules, especially in drugs where enantioselectivity affects potency and safety. Commonly applied in the development of enzyme inhibitors and in structure-activity relationship (SAR) studies.