Used as a protected amino acid derivative in peptide synthesis, enabling selective coupling and preventing unwanted side reactions. The tert-butyl ester and benzyloxycarbonyl (Cbz) protecting groups allow stepwise assembly of peptides with high purity. Commonly employed in solid-phase and solution-phase synthesis of bioactive peptides, pharmaceuticals, and research compounds where controlled deprotection is required. Also utilized in the preparation of peptide-based prodrugs and conjugates due to its stability under various reaction conditions and ease of removal under mild acidic or hydrogenolytic conditions.