Used primarily in peptide synthesis as a protected amino acid derivative, enabling selective deprotection and coupling steps. The Boc group provides temporary protection of the alpha-amino function, while the Dnp (dinitrophenyl) group protects the side chain amine of diaminopropionic acid, allowing orthogonal protection strategies in complex peptide assembly. The Dnp group can be selectively removed under mild conditions using thiols, minimizing side reactions. This compound is especially useful in solid-phase and solution-phase synthesis of structured peptides and peptidomimetics where precise control over reactivity is required. Its application extends to the preparation of labeled peptides and bioconjugates due to the chromophoric nature of the Dnp group, which aids in monitoring reactions.