Used in peptide synthesis as a protected form of histidine, ensuring selective reactions during the construction of complex peptides. It is particularly valuable in solid-phase peptide synthesis (SPPS) where the Boc (tert-butyloxycarbonyl) group protects the amine functionality, while the benzyloxymethyl group shields the imidazole side chain of histidine. This protection prevents unwanted side reactions and allows for precise control over peptide assembly. It is also employed in the development of pharmaceutical compounds and bioactive peptides, where histidine plays a critical role in catalytic or binding activities. Additionally, it serves as an intermediate in the synthesis of modified amino acids for research in biochemistry and drug discovery.