Used primarily in peptide synthesis, this compound serves as a versatile building block for constructing complex peptide chains. It is particularly valuable in solid-phase peptide synthesis (SPPS) due to its dual protection strategy, where the Boc (tert-butyloxycarbonyl) group protects one piperazine nitrogen and the Fmoc (fluorenylmethyloxycarbonyl) group protects the other piperazine nitrogen. This allows for selective deprotection and sequential addition of amino acids, enabling the synthesis of peptides with high precision. Additionally, it is employed in the development of pharmaceuticals, particularly in the creation of peptide-based drugs, where its structural properties facilitate the incorporation of piperazine moieties, often used to enhance bioavailability and pharmacological activity. Its application extends to biochemical research, where it aids in the study of peptide interactions and the design of novel therapeutic agents.