This compound is primarily utilized in peptide synthesis as a protecting group for amino acids. The fluorenylmethoxycarbonyl (Fmoc) group shields the amino group during the synthesis process, preventing unwanted reactions. The 2-nitrophenyl moiety can serve as a photolabile protecting group, allowing for selective deprotection under light exposure. This dual functionality makes it valuable in solid-phase peptide synthesis, where controlled deprotection and coupling steps are essential. Additionally, its structure enables efficient purification and isolation of peptides, ensuring high yields and purity in the final product.