This compound is primarily utilized in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected amino acid derivative, where the fluorenylmethoxycarbonyl (Fmoc) group acts as a temporary protecting group for the amino function, and the tert-butoxycarbonyl (Boc) group protects the indole nitrogen of the tryptophan side chain. This dual protection strategy ensures selective deprotection during the stepwise assembly of peptides, allowing for precise control over the sequence. Its application is crucial in the production of complex peptides and proteins for research, pharmaceuticals, and biotechnology, where high purity and specific sequences are required.