This chemical is primarily used in peptide synthesis, where it serves as a protected form of threonine. The tert-butyl group protects the hydroxyl side chain, while the benzyloxycarbonyl (Cbz) group protects the amino group, preventing unwanted reactions during the synthesis process. It is particularly valuable in solid-phase peptide synthesis (SPPS) and solution-phase peptide synthesis, enabling the stepwise construction of complex peptides with high precision. Additionally, it finds application in the development of pharmaceuticals and bioactive peptides, where controlled deprotection of functional groups is essential for achieving the desired molecular structure. Its use ensures efficient and selective peptide bond formation, making it a key reagent in organic and medicinal chemistry research.