Z-Hyp(Tbu)-OMe is primarily used in peptide synthesis as a protected form of hydroxyproline. It serves as a building block in the construction of complex peptides, particularly in the pharmaceutical and biotechnology industries. The tert-butyl (Tbu) group protects the hydroxyl functionality during synthesis, preventing unwanted reactions, while the methyl ester (OMe) group stabilizes the carboxyl end. This compound is especially valuable in the development of peptide-based drugs, where precise control over amino acid sequences is critical. Its application is also seen in research involving collagen-like peptides, as hydroxyproline is a key component in collagen structure. The use of Z-Hyp(Tbu)-OMe ensures high purity and efficiency in peptide assembly, making it a crucial reagent in advanced synthetic chemistry.