N-Benzoyl-L-tyrosine is widely used in biochemical research as a substrate for studying enzyme activity, particularly in assays involving proteolytic enzymes like chymotrypsin. Its structure makes it suitable for investigating enzyme kinetics and specificity. Additionally, it serves as a key intermediate in the synthesis of peptides and other organic compounds, contributing to advancements in medicinal chemistry and drug development. Its application extends to the development of diagnostic tools and assays for detecting enzyme-related disorders.