Z-Thr-OH is primarily used in peptide synthesis as a protected form of threonine. The Z-group (benzyloxycarbonyl) serves as a protective group for the amino function, preventing unwanted reactions during the peptide coupling process. This compound is particularly useful in solid-phase peptide synthesis (SPPS) and solution-phase synthesis, where selective protection and deprotection of amino acids are crucial. It ensures the correct sequence and structure of the peptide chain, making it valuable in the production of therapeutic peptides, hormones, and research peptides. Additionally, Z-Thr-OH is employed in the study of enzyme mechanisms and protein interactions due to its stability and ease of handling in laboratory settings.