N-tert-Butylglycine hydrochloride is widely used in peptide synthesis as a building block for creating modified peptides. Its tert-butyl group provides steric hindrance, which helps in controlling the conformation and stability of the resulting peptides. This makes it valuable in the development of peptide-based drugs and bioactive molecules. Additionally, it is utilized in research to study enzyme-substrate interactions and protein folding due to its unique structural properties. The compound also finds applications in the preparation of chiral catalysts and ligands for asymmetric synthesis, enhancing the efficiency and selectivity of chemical reactions. Its role in medicinal chemistry is notable for designing inhibitors and modulators of biological targets.