N-Acetylglycine ethyl ester is primarily used in organic synthesis as a building block for the preparation of more complex molecules. It serves as an intermediate in the production of pharmaceuticals, particularly in the synthesis of peptides and other bioactive compounds. The ester group in the molecule makes it a useful precursor for creating modified amino acids, which are essential in drug development and biochemical research. Additionally, it is employed in the study of enzymatic reactions and metabolic pathways, providing insights into the behavior of acetylated amino acids in biological systems. Its role in facilitating the formation of specific chemical bonds makes it valuable in the design and synthesis of targeted therapeutic agents.