N-Acetyl-L-serine is primarily used in biochemical research and pharmaceutical applications. It serves as a building block in peptide synthesis, aiding in the creation of complex molecules for drug development. This compound is also utilized in studies related to amino acid metabolism and protein structure, helping researchers understand cellular processes. Additionally, it finds application in the production of cosmetic formulations, where it acts as a moisturizing agent and skin conditioner due to its hydrating properties. Its role in enhancing skin barrier function makes it valuable in skincare products.