Boc-His(1-Me)-OH is widely used in peptide synthesis as a protected form of histidine. Its primary application is in the preparation of peptides where histidine needs to be selectively incorporated without interference from its side chain reactivity. The Boc (tert-butoxycarbonyl) group protects the amino group, while the 1-methyl modification stabilizes the imidazole ring, making it suitable for solid-phase peptide synthesis (SPPS). This compound is particularly valuable in synthesizing peptides for biochemical research, drug development, and the study of protein interactions. It ensures high yield and purity in peptide chains, especially in complex sequences where histidine plays a critical role. Additionally, it is used in the development of peptide-based therapeutics, including antimicrobial peptides and enzyme inhibitors.