L-Asparagine tert-butyl ester is primarily used in peptide synthesis as a protecting group for the carboxyl function of asparagine. This compound helps in preventing unwanted side reactions during the formation of peptide bonds, ensuring the integrity and specificity of the peptide chain being synthesized. It is particularly valuable in solid-phase peptide synthesis (SPPS), where selective deprotection is required to build complex peptides step-by-step. Additionally, its tert-butyl ester group can be easily removed under mild acidic conditions, making it a convenient choice for researchers in biochemistry and pharmaceutical development. Its application extends to the production of bioactive peptides and therapeutic agents, where precise control over molecular structure is crucial.