Used primarily in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. It serves as a protected amino acid derivative, where the Fmoc (fluorenylmethyloxycarbonyl) group acts as a temporary protecting group for the N-methylated alpha-amine functionality. This allows for the sequential addition of amino acids to build peptides with high precision. The N-methyl group on the alpha-amino nitrogen can restrict backbone flexibility, influence the peptide's conformation, and enhance biological activity or stability (e.g., resistance to proteolysis), making it valuable in the design of peptides with specific structural or functional properties. Commonly applied in pharmaceutical research for developing peptide-based drugs, as well as in biochemical studies to explore protein-protein interactions and enzyme mechanisms.