Fmoc-Phe(2,6-DiF)-OH is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected amino acid derivative, where the Fmoc (fluorenylmethyloxycarbonyl) group acts as a temporary protecting group for the amine functionality. This allows for the sequential addition of amino acids to build peptides with precise sequences. The presence of the 2,6-difluoro substitution on the phenylalanine side chain introduces specific properties, such as enhanced stability or altered binding affinity, making it valuable in the design of peptides for biochemical and pharmaceutical research. It is often employed in the development of peptide-based drugs, enzyme inhibitors, and probes for studying protein-protein interactions.