Boc-His-OH is widely used in peptide synthesis as a protected form of histidine. It serves as a crucial building block in the production of peptides and proteins, ensuring the histidine residue remains stable during the synthesis process. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it helps in the stepwise assembly of amino acids. Additionally, Boc-His-OH is employed in the development of pharmaceuticals, including drugs targeting enzyme inhibition and receptor modulation, due to histidine's role in catalytic and binding sites. Its application extends to research in biochemistry and molecular biology, where it aids in studying protein structure and function.