Boc-Ile-OH is widely used in peptide synthesis as a protective agent for the amino group of isoleucine. It plays a crucial role in the stepwise construction of peptides, ensuring that specific amino acids are selectively activated and deprotected during the process. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it helps in building complex peptide chains with high precision. Additionally, it is employed in the production of pharmaceuticals, where controlled peptide synthesis is essential for creating biologically active compounds. Its stability and ease of removal under mild acidic conditions make it a preferred choice in organic and medicinal chemistry research.