Fmoc-Tpi-OH is widely used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a key building block for introducing the Tpi (Tetrahydroisoquinoline-3-carboxylic acid) moiety into peptide sequences. This compound is essential for creating peptides with specific structural and functional properties, often utilized in the development of bioactive peptides and peptidomimetics. Its Fmoc (Fluorenylmethyloxycarbonyl) protecting group ensures selective deprotection during synthesis, enabling precise control over the assembly of complex peptide chains. Applications extend to drug discovery, where it aids in designing peptide-based therapeutics, enzyme inhibitors, and receptor ligands. Additionally, it is employed in research to study peptide interactions, folding, and stability, contributing to advancements in biochemistry and medicinal chemistry.