Tosyl-D-valine is primarily used in organic synthesis as a chiral building block for the preparation of peptides and other biologically active compounds. Its tosyl group acts as a protective group for the amino functionality, allowing selective reactions to occur at other sites of the molecule. This compound is particularly valuable in the synthesis of enantiomerically pure substances, which are crucial in the development of pharmaceuticals, such as antibiotics and antiviral drugs. Additionally, Tosyl-D-valine is employed in research settings to study enzyme mechanisms and protein interactions, due to its ability to mimic natural amino acids while providing additional reactivity for chemical modifications.