Pronase E is widely utilized in various biochemical and biotechnological applications due to its broad specificity and ability to degrade proteins into smaller peptides and amino acids. In molecular biology, it is often employed for cell dissociation and tissue disaggregation, facilitating the isolation of individual cells for culture or analysis. The enzyme is also used in the preparation of DNA and RNA by removing proteins that may interfere with nucleic acid extraction processes. Additionally, Pronase E plays a role in studying protein structure and function, as it can cleave peptide bonds at multiple sites, aiding in the analysis of protein sequences and interactions. In the pharmaceutical industry, it is used in the development of therapeutic proteins and peptides. Furthermore, Pronase E is applied in the food industry to modify protein textures and enhance flavors in certain products. Its versatility and effectiveness make it a valuable tool in both research and industrial settings.