Prolidase, sourced from porcine kidney, is widely utilized in biochemical research for its ability to hydrolyze dipeptides containing proline or hydroxyproline at the C-terminus. This enzyme plays a critical role in studying collagen metabolism, as it helps break down proline-containing peptides, which are essential components of collagen. It is also employed in clinical diagnostics to assess prolidase deficiency, a rare genetic disorder that affects collagen turnover and leads to various skin and systemic abnormalities. Additionally, prolidase is used in biotechnology for protein sequencing and peptide synthesis, enabling the precise analysis and creation of peptide structures. Its application extends to the development of therapeutic agents targeting conditions related to collagen degradation and wound healing.