Carboxypeptidase A, derived from bovine pancreas, is widely utilized in biochemical research and industrial applications due to its ability to cleave peptide bonds. In protein sequencing, it is employed to analyze the C-terminal amino acids of proteins, aiding in the determination of protein structure and function. This enzyme is also used in the food industry to enhance flavor development by breaking down proteins into smaller peptides and amino acids, which contribute to taste and aroma. Additionally, it plays a role in the production of bioactive peptides with potential health benefits. In biotechnology, Carboxypeptidase A is applied in the modification of therapeutic proteins to improve their stability and efficacy. Its specificity and efficiency make it a valuable tool in both scientific and commercial settings.