Carboxypeptidase Y is widely used in biochemical research for protein sequencing and peptide mapping. It specifically cleaves peptide bonds at the C-terminal end of amino acids, making it valuable for analyzing protein structures. This enzyme is particularly useful in proteomics for identifying post-translational modifications and characterizing protein fragments. Additionally, it is employed in the production of bioactive peptides and in studies involving enzyme kinetics and specificity. Its ability to work under a broad range of pH conditions enhances its versatility in various experimental setups.