Cathepsin B from bovine spleen is widely utilized in biochemical research for its proteolytic activity, particularly in studying protein degradation and turnover. It plays a crucial role in understanding lysosomal function and its implications in diseases such as cancer, arthritis, and neurodegenerative disorders. Researchers use it to investigate the mechanisms of intracellular protein breakdown and to develop inhibitors for therapeutic applications. Additionally, it is employed in tissue processing and antigen retrieval techniques in histology, aiding in the analysis of cellular structures. Its ability to cleave specific peptide bonds makes it valuable in peptide mapping and protein sequencing studies.