Dispase II is widely used in cell culture and tissue engineering for its ability to dissociate tissues into single cells without causing significant damage. It is particularly effective in isolating epithelial cells, making it valuable in research involving skin, kidney, and other epithelial tissues. In regenerative medicine, Dispase II is employed to harvest cells for transplantation and to create cell sheets for tissue repair. It is also utilized in the study of cell adhesion and migration, as it selectively cleaves specific proteins in the extracellular matrix. Additionally, Dispase II plays a role in organoid culture, enabling the separation and propagation of stem cells for 3D tissue modeling. Its gentle enzymatic activity ensures high cell viability, making it a preferred choice in various biomedical applications.