This chemical is widely used in biochemical research as a fluorogenic substrate for caspase-3 and related caspases, such as caspase-7. It is particularly valuable in apoptosis studies, where it helps detect and measure caspase activity, a key indicator of programmed cell death. Researchers use it to monitor enzyme kinetics and screen for potential inhibitors or activators of caspases. Its application extends to drug discovery and development, where it aids in evaluating the efficacy of therapeutic agents targeting apoptosis pathways. Additionally, it is employed in cell-based assays to study the mechanisms of cell death in various diseases, including cancer and neurodegenerative disorders.