Azocasein is widely used as a substrate in enzymatic assays, particularly for proteolytic enzymes like proteases. Its application is crucial in biochemistry and molecular biology research, where it helps in determining enzyme activity and kinetics. The compound's structure allows it to be cleaved by proteases, producing a measurable change that can be quantified spectrophotometrically. This makes it a valuable tool in studying enzyme mechanisms, screening protease inhibitors, and evaluating the efficiency of enzymatic reactions. Additionally, azocasein is employed in industrial settings to test the effectiveness of proteases in detergents, food processing, and other applications where protein degradation is essential.