Benzoylglycylglycine is primarily used in biochemical research as a substrate for studying enzyme activity, particularly in assays involving proteases and peptidases. It serves as a model compound to understand the hydrolysis mechanisms of peptide bonds, aiding in the development of enzyme inhibitors and therapeutic agents. Additionally, it is employed in the synthesis of more complex peptides and in the investigation of metabolic pathways related to peptide degradation. Its stability and specificity make it a valuable tool in enzymology and pharmaceutical research.