Alanylleucylglycine is primarily utilized in biochemical research as a model peptide to study peptide interactions, enzyme specificity, and protein folding mechanisms. It serves as a substrate in enzymatic assays to investigate the activity of proteases and peptidases, helping to understand their catalytic behavior and substrate preferences. Additionally, it is employed in peptide synthesis studies to explore efficient coupling methods and optimize reaction conditions. In cellular studies, it can be used to examine peptide transport across membranes and its role in nutrient uptake. Its simple structure makes it a valuable tool in teaching and training settings for demonstrating fundamental peptide chemistry concepts.