Z-Gly-Gly-Arg-AMC is widely used in biochemical research as a fluorogenic substrate for protease assays. It is particularly valuable for studying the activity of enzymes like trypsin, thrombin, and other serine proteases. When the enzyme cleaves the substrate, it releases the fluorescent AMC (7-amino-4-methylcoumarin) moiety, allowing researchers to quantify enzyme activity through fluorescence measurement. This application is crucial in understanding enzyme kinetics, inhibitor screening, and characterizing protease function in various biological processes. Additionally, it is used in drug discovery to evaluate the efficacy of protease inhibitors, making it a key tool in both basic research and pharmaceutical development.