Bestatin trifluoroacetate is widely used in biochemical research as a potent inhibitor of aminopeptidases, particularly leucine aminopeptidase. It is employed to study enzyme mechanisms and to explore the role of aminopeptidases in various biological processes, including protein metabolism and immune response modulation. In immunology, it is utilized to investigate its effects on enhancing immune cell activity, particularly in the context of cancer immunotherapy. Additionally, it serves as a tool compound in drug discovery to identify and validate new therapeutic targets related to aminopeptidase activity. Its application extends to studying inflammation and immune regulation, providing insights into potential treatments for autoimmune diseases and infections.