Cysteine protease inhibitors are widely used in biomedical research to study the role of cysteine proteases in various biological processes. They are essential tools in understanding diseases like cancer, neurodegenerative disorders, and parasitic infections, where these enzymes play a critical role. In drug development, these inhibitors serve as potential therapeutic agents, particularly in targeting conditions such as osteoporosis, rheumatoid arthritis, and viral infections like COVID-19, where cysteine proteases are involved in disease progression. Additionally, they are utilized in the food industry to prevent protein degradation, ensuring the quality and shelf life of products. In agriculture, these inhibitors help in developing pest-resistant crops by targeting proteases essential for insect survival.