Acetyl-pepstatin is widely used in biochemical research as a potent inhibitor of aspartic proteases, including pepsin, cathepsin D, and renin. Its application is particularly valuable in studying the role of these enzymes in various biological processes, such as protein degradation and cellular signaling pathways. Researchers utilize acetyl-pepstatin to block protease activity in experimental setups, enabling the investigation of enzyme function and the development of therapeutic strategies targeting protease-related diseases. Additionally, it is employed in protein purification processes to prevent unwanted proteolysis, ensuring the integrity of proteins during isolation and analysis. Its specificity and effectiveness make it a crucial tool in enzymology and drug discovery research.