Antipain dihydrochloride is widely used in biochemical and cellular research as a protease inhibitor. It effectively inhibits serine and cysteine proteases, making it valuable in studying enzyme mechanisms and protein interactions. In cell culture studies, it helps prevent protein degradation, ensuring accurate analysis of cellular processes. Additionally, it is employed in protein purification protocols to maintain protein integrity during extraction and isolation. Its role in inhibiting proteases also aids in investigating apoptosis and other protease-dependent pathways in biological systems.