Calpain Inhibitor II is widely used in biochemical research to study the role of calpain proteases in cellular processes. It is particularly valuable in investigating cell death mechanisms, such as apoptosis and necrosis, as calpain activation is often associated with these pathways. Researchers use this inhibitor to block calpain activity, allowing them to isolate its effects and better understand its involvement in diseases like neurodegenerative disorders, muscular dystrophy, and ischemia-reperfusion injury. Additionally, it is employed in studies of cytoskeletal remodeling, as calpains play a key role in the cleavage of structural proteins. Its application extends to drug development, where it serves as a tool to validate calpain as a therapeutic target.