Amastatin hydrochloride is primarily used in biochemical research as a potent inhibitor of aminopeptidases, enzymes that cleave amino acids from the N-terminus of proteins or peptides. Its application is crucial in studying the role of these enzymes in various biological processes, including protein degradation, antigen presentation, and peptide hormone regulation. Researchers utilize it to investigate the mechanisms of enzyme activity and to develop potential therapeutic strategies targeting aminopeptidase-related diseases. Additionally, it is employed in cell culture studies to modulate peptide processing and understand its impact on cellular functions. Its inhibitory properties make it a valuable tool in proteomics and enzymology research.