Pisatin is a phytoalexin produced by pea plants in response to fungal infection, particularly from pathogens like Nectria haematococca. Its primary application lies in plant defense mechanisms, where it acts as a natural antimicrobial agent to inhibit the growth of invading fungi. Due to its antifungal properties, pisatin plays a key role in studying plant-pathogen interactions and resistance mechanisms in agriculture. Researchers use pisatin as a model compound to understand how plants develop biochemical defenses, which aids in breeding disease-resistant crop varieties. Additionally, it serves as a reference in biochemical studies exploring detoxification strategies employed by fungal pathogens, contributing to the development of more effective plant protection strategies.