Hygromycin B is widely used in molecular biology and genetic research as a selective agent. It is particularly effective in selecting and maintaining eukaryotic cells that have been transformed with a plasmid containing the hygromycin resistance gene. This antibiotic is commonly employed in the selection of stable cell lines, ensuring that only cells with the desired genetic modification survive. Additionally, it is utilized in plant biotechnology to select transgenic plants that have incorporated the hygromycin resistance gene. Its application extends to the study of gene function and expression, as well as the development of genetically modified organisms (GMOs) in both plant and animal systems.