Bisindolylmaleimide XI hydrochloride is primarily utilized in biochemical research as a potent and selective inhibitor of protein kinase C (PKC). It is widely employed in studies aimed at understanding the role of PKC in various cellular processes, including signal transduction, cell proliferation, and apoptosis. Researchers use this compound to investigate the mechanisms underlying diseases where PKC activity is dysregulated, such as cancer, cardiovascular disorders, and neurodegenerative conditions. Its ability to inhibit PKC isoforms makes it a valuable tool for dissecting specific pathways and evaluating potential therapeutic targets. Additionally, it is used in pharmacological studies to assess the efficacy of PKC inhibitors in preclinical models.