Trans-Aconitic acid is primarily used in the field of biochemistry and industrial applications. It serves as an intermediate in the tricarboxylic acid (TCA) cycle, playing a role in cellular respiration and energy production. In industrial contexts, it is utilized as a chelating agent, helping to bind and remove metal ions in processes like water treatment and cleaning formulations. Additionally, it finds use in the synthesis of certain polymers and resins, contributing to their stability and performance. In agriculture, it can act as a natural herbicide, inhibiting the growth of unwanted plants. Its applications also extend to the food industry, where it may be used as an acidulant or preservative to enhance flavor and shelf life. Furthermore, it is employed in biochemical and pharmacological research to study cellular mechanisms and develop new pharmaceuticals.