Scopolin is primarily used in pharmaceutical research due to its biological activity. It serves as a precursor or marker compound in the study of plant-derived metabolites with anticholinergic properties. Its presence in certain medicinal plants makes it valuable for quality control in herbal medicine production. Scopolin is also investigated for its potential antioxidant and anti-inflammatory effects, supporting its role in developing natural health products. Additionally, it is used as a reference standard in analytical methods like HPLC for identifying related compounds in complex plant extracts.